COMPUTATIONAL DESIGN OF NOVEL CAS9 PAM-INTERACTING DOMAINS USING EVOLUTION-BASED MODELLING AND STRUCTURAL QUALITY ASSESSMENT.

We present here an approach to protein design that combines (i) scarce functional information such as experimental data (ii) evolutionary information D-Sub/Serial learned from a natural sequence variants and (iii) physics-grounded modeling.Using a Restricted Boltzmann Machine (RBM), we learn a sequence model of a protein family.We use semi-supervis

BackgroundExisting instruments that assess individuals’ relationships with mobile phones tend to focus on negative constructs such as addiction or dependence, and appear to assume that high mobile phone use reflects pathology.Mobile phones can be beneficial for health behavior change, disease management, work Electronic Hood Lock productivity, an